BIO251 Laboratory Images, Videos, and Study Material


Video demonstration of aseptic technique (.mpg file)

Video of how to effectively use your compound light microscope (.mpg file)

Video of smear preparation and simple staining (.mpg file)


The images below are available as a study tool for the laboratory section of the BIO251: Medical and Public Health Microbiology course. 

Lab EXAM 1 prep material - Microscopic images of various bacteria under various staining techniques and media-based tests we have used.  For the microscope images, you MUST know the species, by name, and their associated traits learned from the staining techniques.  For the biochemical-based tests, DO NOT memorize which species is positive or negative for each biochemical test.  Rather, as you study focus on how a test is performed, what you learn about an organism from that test, and how a positive result is detected using the appropriate media/reagents.

Click on a thumbnail image to view full-size.

Gram stain of Escherichia coli

Gram stain of a Bacillus species

Gram stain of Staphylococcus aureus

An endospore stain of Bacillus cereus using malachite green

Capsule stain of bacilli bacteria

Acid-Fast stain of a Mycobacterium species

Beta hemolysis (left) and alpha hemolysis (right) on a blood agar plate

Mannitol salt agar plate demonstrating mannitol fermentation and non-fermentation

A lactose fermenter and a non-lactose fermenter demonstrated on EMB

 Starch Hydrolysis performed on a starch agar plate

Casein hydrolysis demonstrated on a skim milk agar plate

 

Carbohydrate Fermentation of glucose, lactose, and sucrose

Urease activity demonstrated in a urea broth

 

 

    



Lab EXAM 2 prep material - These images are labeled only with the name of the biochemical test and the media that test is being performed in/on.  It is NOT the goal of this course to have you memorize which species is positive for which biochemical test.  Rather, as you study focus on how a test is performed, what you learn about an organism from that test, and how a positive result is detected using the appropriate media/reagents.

Click on a thumbnail image to view full-size.

Kirby-Bauer disc diffusion test for antibiotic susceptibility

Citrate utilization demonstrated on a Simmon's citrate slant



Lysine decarboxylation as demonstrate in an lysine decarboxylase broth

 

 

Methyl Red test performed in a glucose-rich MRVP broth

 



Hydrogen Sulfide, Indole production, motility demonstrated in a SIM tube

 

Phenylalanine Deaminase activity demonstrated on a Phenylalanine agar slant

 

Carbohydrate utilization demonstrated on triple sugar iron (TSI) agar slants

 

Snyder test agar for dental caries

(image from http://microbiology2420.blogspot.com/2009_04_01_archive.html)

Voges-Proskauer test performed in a glucose-rich MRVP broth